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KMID : 1094020160330040194
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Journal of Veterinary Clinics
2016 Volume.33 No. 4 p.194 ~ p.199
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Effect of trans-10, cis-12 Conjugated Linoleic Acid on Production of Prostaglandin E2, Cyclooxygenase-2 and 5-lipoxygenase in Lipopolysaccharide-Stimulated Porcine Peripheral Blood Mononuclear Cells
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Seo Hae-Ryun
Ahn Chang-Hwan
Kang Byeong-Teck
Kang Ji-Houn
Jeung Eui-Bae
Yang Mhan-Pyo
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Abstract
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The objective of this study was to examine the effect of trans-10, cis-12 conjugated linoleic acid (t10c12-CLA) on the expression of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) pathway in lipopolysaccharide(LPS)-stimulated porcine peripheral blood mononuclear cells (PBMCs). t10c12-CLA was treated with differentconcentrations in culture medium of LPS-naive and LPS-stimulated PBMCs. The mRNA expressions of prostaglandinE2 (PGE2)-synthase, COX-2 and 5-LOX were measured using quantitative real-time PCR. In addition, the productionlevels of PGE2 and 5-LOX in culture supernatant from PBMCs with or without LPS were assessed by ELISA. InLPS-naive PBMCs, treatment of t10c12-CLA significantly (p < 0.05) increased the mRNA expressions of PGE2 synthaseand 5-LOX compared to vehicle control. Expression of COX-2 mRNA did not show significant difference comparedto vehicle control by t10c12-CLA treatment in LPS-naive PBMCs. However, the addition of LPS in PBMCs markedly(p < 0.05) increased the mRNA expression of COX-2, PGE2 synthase and 5-LOX, and also significantly (p < 0.05)enhanced the production of PGE2 and 5-LOX relative to LPS-naive PBMCs, respectively. However, the addition oft10c12-CLA significantly (p < 0.01) suppressed the LPS-induced excessive expression of COX-2, PGE2 synthase, and5-LOX compared to those of PBMCs treated with LPS alone. The production levels of PGE2 and 5-LOX in culturesupernatant from LPS-stimulated PBMCs were also significantly (p < 0.05) inhibited by the treatment of t10c12-CLAcompared to LPS alone. These results suggested that t10c12-CLA has an anti-inflammatory effect via dual inhibitionof COX-2 and 5-LOX with gene expression and production level in LPS-stimulated porcine PBMCs. Therefore, itwas thought that t10c12-CLA can attenuate the inflammatory response by down-regulation of eicosanoids production.
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KEYWORD
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10, cis-12-conjugated linoleic acid, COX-2, PGE2, 5-LOX, lipopolysaccharide, pigs
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